痕量H2O2的酶催化-阳离子表面活性剂缔合物微粒共振散射光谱测定

在醋酸盐缓冲溶液中, 辣根过氧化物酶(HRP)催化H2O2与过量的I－反应生成 , 分别与阳离子表面活性剂(CS) 十四烷基苄基二甲基氯化铵(TDMAC)、十二烷基苄基二甲基氯化铵(DDAC)、十六烷基三甲基溴化铵(CTAB)、十八烷基苄基二甲基氯化铵(ODAC)、氯代十六烷基吡啶(CPC)、氯代十二烷基吡啶(DPC)和四丁基碘化铵(TBAI)形成TDMAC-I3, DDAC-I3, CTAB-I3, ODAC-I3, CPC-I3, DPC-I3和TBAI-I3缔合物微粒. 该缔合物微粒均在460 nm处有一个较强的共振散射峰. H2O2浓度在0.17×10－7～173×10－7, 0.43×10－7～173×10－7, 1.73×10－7～259×10－7, 1.73×10－7～86.4×10－7, 1.73×10－7～216×10－7, 0.86×10－7～259×10－7和0.86×10－7～86.4×10－7 mol•L－1范围内分别与各体系在468 nm处的共振光谱强度呈线性关系, 其检出限分别为0.86×10－8, 2.2×10－8, 8.6×10－8, 4.6×10－8, 3.6×10－8, 4.3×10－8和4.4×10－8 mol•L－1. 本文将TDMAC体系用于过氧化氢测定, 结果满意.

Horseradish Peroxidase Catalyzed-Resonance Scattering Spectral Determination of Trace H2O2 Using Cationic Surfactant

In acetate acid buffer solution, horseradish peroxidase (HRP) catalyzed H2O2 oxidation of excess I－ to form , and combined with cationic surfactant (CS) such as tetradecyl dimethylbenzyl ammonium chloride (TDMAC), dodecyl dimethylbenzyl ammonium chloride (DDAC), cetyltrimethyl ammonium bromide (CTAB), octadecyl dimethyl benzyl ammonium chloride (ODAC), cetylpyridinium chloride (CPC), dodecyl pyridinium chloride (DPC) and tetrabutyl ammonium iodide (TBAI) to produce TDMAC-I3, DDAC-I3, CTAB-I3, ODAC-I3, CPC-I3, DPC-I3 and TBAI-I3 association particles, respectively, that exhibited the strongest resonance scattering peak at 468 nm. The H2O2 concentration in the range of 0.17×10－7～173×10－7, 0.432×10－7～173×10－7, 1.73×10－7～259×10－7, 1.73×10－7～86.4×10－7, 1.73×10－7～216×10－7, 0.86×10－7～259×10－7 and 0.86×10－7～86.4×10－7 mol•L－1, was linear to the intensity at 460 nm, with a detection limit of 0.86×10－8, 2.2×10－8, 8.6×10－8, 4.6×10－8, 3.6×10－8, 4.3×10－8 and 4.4×10－8 mol•L－1, respectively. The TDMAC resonance scattering spectral method was used to the determination of H2O2 in real samples, with satisfactory results.