Qualitative and quantitative analysis of uric acid,creatine and creatinine together with carbohydrates in biological material by HPTLC |
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Authors: | R Klaus W Fischer H E Hauck |
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Institution: | (1) Fichtestrasse 25, 6100 Darmstadt, Germany;(2) E. Merck, P.O. Box 4119, 6100 Darmstadt, Germany |
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Abstract: | Summary In clinical diagnosis creatine, creatinine and uric acid are important parameters for the evaluation of renal diseases, and
are partially responsible for gout and the formation of renal calculi. The determination of various carbohydrates, especially
glucose, in urine and serum serves as an indicator of diabetes and other carbohydrate anomalies. A simple, rapid and economic
method for the simultaneous screening and quantitation of these compounds is presented. No sample preparation is necessary
for the determination in urine or in serum. The proposed method consists of separations on an amino modified HPTLC precoated
plate. The detection of all relevant substances is reproducibly performed by simply heating the chromatogram to give stable
fluorescent derivatives. The detection limits in all cases lie significantly below the physiological range. |
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Keywords: | Thin-layer chromatography Amino modified silica Creatine creatinine uric acid and carbohydrates Fluorescence detection In-situ densitometry |
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