|
|||||
|
|
Determination of 20(S)-protopanaxadiol in rat plasma by LC-MS/MS and its application to the pharmacokinetic study: A comparative study of its solution and two oral formulations |
|
| Authors: | Meihua Han Jing Chen Yuqin Wang Shilin Chen Xiangtao Wang |
| Affiliation: | 19933. Chinese Academy of Medical Sciences & Peking Union Medical College Institute of Medicinal Plant Development, No. 151 Malianwa North Road, Haidian District, Beijing, 100193, China 29933. Life Science and Environment Science Research Center, Engineering Research Center of Natural Anticancer Drugs, Ministry of Education Harbin, Harbin University of Commerce, Harbin, 150076, China 39933. Shanghai Innovative Research Center of Traditional Chinese Medicine (SIRC/TCM), Shanghai, 201203, China |
| Abstract: | A high-performance liquid chromatography — tandem mass spectrometry method was used in a comparative pharmacokinetic study on 20(S)-protopanaxadiol in its solution, pharmacosomes and hydroxy-propyl-β-cyclodextrin inclusion compound. The calibration linearity range was 2.5–5000 ng/mL and the limit of quantification was 2.5 ng/mL. The intra- and inter-assay coefficients of variation were less than 7% and the recoveries were 67.4, 63.3 and 69.6% at 5, 250 and 4500 ng/mL. In contrast to 20(S)-protopanaxadiol solution, its pharmacosomes and hydroxypropyl-β-cyclodextrin inclusion compound improved the oral bioavailability to different extent and meanwhile were much safer in clinical use. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |