Fluorescence near-field microscopy of DNA at sub-10 nm resolution |
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Authors: | Ma Ziyang Gerton Jordan M Wade Lawrence A Quake Stephen R |
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Institution: | Applied Physics and Physics, California Institute of Technology, Pasadena, California 91125, USA. |
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Abstract: | We demonstrate apertureless near-field microscopy of single molecules at sub-10 nm resolution. With a novel phase filter, near-field images of single organic fluorophores were obtained with approximately sixfold improvement in the signal-to-noise ratio. The improvement allowed pairs of molecules separated by approximately 15 nm to be reliably and repeatedly resolved, thus demonstrating the first true Rayleigh resolution test for near-field images of single molecules. The potential of this technique for biological applications was demonstrated with an experiment that measured the helical rise of A-form DNA. |
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