Construction of CdSe/ZnS quantum dot microarray in a microfluidic chip |
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Authors: | HongWei Liu YingYing Jing Xu Yu DaiWen Pang ZhiLing Zhang |
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Institution: | 1Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education); College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China 2Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China |
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Abstract: | Microarray technology has been proved to be greatly helpful for biomedical and biological diagnosis. And the evaluation of its biological applications lies in the detection sensitivity, which requires high intensity and stability of the signal. Recently, several nanomaterials, especially semiconductor nanomaterials, due to their excellent fluorescence properties, have been widely used to construct microarrays for biosensors. Here, we presented an approach for constructing CdSe/ZnS quantum dot (QD) microarray in microfluidic channels on a glass slide by photolithography. The conditions for immobilizing stable and uniform QD microarray on the glass slide were optimized. Several types of QD microarrays with different emission wavelengths and modified groups were constructed using silanization and lithography technology. Based on the fluorescence quenching effect of Cu2+ on QDs, the microfluidic chip with QD microarray was applied for the determination of Cu2+. 1 nmol/L Cu2+ could be detected by this method. |
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Keywords: | microarray quantum dots microfluidic Cu2+ |
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