Quantitative thin-layer chromatographic determination of dexamethasone and dexamethasone sodium hydrogen sulfate in blood and in pharmaceutical preparations

Summary Quantitative, thin-layer Chromatographic method for the determination of dexamethasone and dexamethasone sodium hydrogen sulfate in the nanogram range in blood and in pharmaceutical preparations is described. The extraction of both active ingredients was performed in a fully automated, electronically controlled extraction apparatus within 15 min. Dexamethasone and dexamethasone sodium hydrogen sulfate were separated on pre-coated silica gel 60F254 plates and measured directly on the plates by reflectance at _max = 245 nm and 243 nm, respectively. The extraction and Chromatographic methods are suitable for the determination of these drugs in pharmaceutical preparations and in biological materials. Furthermore, the described methods are suitable for pharmacokinetic and bioavailability studies as they enable the determination of 50 ng of the drugs with a coefficient of variation of 7% and 9% for dexamethasone sodium hydrogen sulfate and dexamethasone, respectively.

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Quantitative dünnschicht-chromatographische Bestimmung von Dexamethason und Dexamethason-Natriumhydrogensulfat in Blut und pharmazeutischen Präparaten