| HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method |
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| 引用本文： | ZeYiYAN ChengJinJIAO FengMinLI YongMinLIANG ZhiXiaoLI. HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method[J]. 中国化学快报, 2005, 16(5): 639-642 |
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| 作者姓名： | ZeYiYAN ChengJinJIAO FengMinLI YongMinLIANG ZhiXiaoLI |
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| 作者单位： | [1]StateKeyLaboratoryofAppliedOrganicChemistry,LanzhouUniversity,Lanzhou730000 [2]SchoolofLifeScience,LanzhouUniversity,Lanzhou730000 |
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| 摘 要： | An assay is presented for simultaneously determining 5 biogenic amines and the major non-protein amino acids: the toxin β-N-oxalyl-L-α，β-diaminopropanoic acid (β-ODAP), its isomer α-ODAP and homoarginine in Lathyrus sativus extracts using the HPLC system after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-Cl). However, it is more worthy of noting that this paper also describes a new extraction method using 0.2 mol/L HClO4. The new method has some advantages: shorter extraction-time, simultaneous extraction of free amino acids and polyamines, better inhibiting the isomerization of β-ODAP to α-ODAR and so on.
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| 关 键 词： | 生物胺 萃取法 山黧豆 蛋白质 氨基酸 异构体 |
HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method |
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| Ze Yi YAN,Cheng Jin JIAO,Feng Min LI,Yong Min LIANG,Zhi Xiao LI State Key Laboratory of Applied Organic Chemistry,Lanzhou University,Lanzhou School of Life Science,Lanzhou University,Lanzhou. HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method[J]. Chinese Chemical Letters, 2005, 16(5): 639-642 |
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| Authors: | Ze Yi YAN Cheng Jin JIAO Feng Min LI Yong Min LIANG Zhi Xiao LI State Key Laboratory of Applied Organic Chemistry Lanzhou University Lanzhou School of Life Science Lanzhou University Lanzhou |
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| Affiliation: | Ze Yi YAN1,Cheng Jin JIAO2,Feng Min LI2,Yong Min LIANG1,Zhi Xiao LI1 1State Key Laboratory of Applied Organic Chemistry,Lanzhou University,Lanzhou 730000 2School of Life Science,Lanzhou University,Lanzhou 730000 |
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| Abstract: | |
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| Keywords: | ODAP homoginine HPLC biogenic amines new extraction method. |
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