Recent developments in microarray-based enzyme assays: from functional annotation to substrate/inhibitor fingerprinting |
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| Authors: | Hongyan Sun Souvik Chattopadhaya Jun Wang Shao Q Yao |
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| Institution: | (1) Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543 Singapore, Singapore;(2) Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, 117543 Singapore, Singapore;(3) Medicinal Chemistry Program of the Office of Life Sciences, National University of Singapore, 3 Science Drive 3, 117543 Singapore, Singapore |
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| Abstract: | Recent advances in proteomics have provided impetus towards the development of robust technologies for high-throughput studies
of enzymes. The term “catalomics” defines an emerging ‘-omics’ field in which high-throughput studies of enzymes are carried
out by using advanced chemical proteomics approaches. Of the various available methods, microarrays have emerged as a powerful
and versatile platform to accelerate not only the functional annotation but also the substrate and inhibitor specificity (e.g.
substrate and inhibitor fingerprinting, respectively) of enzymes. Herein, we review recent developments in the fabrication
of various types of microarray technologies (protein-, peptide- and small-molecule-based microarrays) and their applications
in high-throughput characterizations of enzymes. |
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| Keywords: | Enzyme assay Microarray Catalomics Activity-based probe Activity-based fingerprinting Inhibitor fingerprinting Drug discovery |
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