A Two-Photon Fluorescent Probe for the Visual Detection of Peroxynitrite in Living Cells and Zebrafish |
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| Authors: | Zhencai Xu Jin Qian Yufeng Ge Yalan Wang Hongwei Chen |
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| Affiliation: | 1.Guanyun People’s Hospital, Lianyungang 222000, China;2.Modern Education Technology Center, Department of Critical Care Medicine, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou 571199, China;3.Department of Emergent Trauma Surgery, Qilu Hospital of Shandong University, Jinan 250012, China; |
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| Abstract: | Peroxynitrite (ONOO−), as an important reactive oxygen species (ROS), holds great potential to react with a variety of biologically active substances, leading to the occurrence of various diseases such as cancer and neurodegenerative diseases. In this work, we developed a novel mitochondria-localized fluorescent probe, HDBT-ONOO−, which was designed as a mitochondria-targeting two-photon fluorescence probe based on 1,8-naphthylimide fluorophore and the reactive group of 4-(bromomethyl)-benzene boronic acid pinacol ester. More importantly, the probe exhibited good biocompatibility, sensitivity, and selectivity, enabling its successful application in imaging the generation of intracellular and extracellular ONOO−. Furthermore, exogenous and endogenous ONOO− products in live zebrafish were visualized. It is greatly expected that the designed probe can serve as a useful imaging tool for clarifying the distribution and pathophysiological functions of ONOO− in cells and zebrafish. |
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| Keywords: | peroxynitrite fluorescent probe cell imaging zebrafish |
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