Electrophoretic investigation of the histones of the cotton plant in polyacrylamide gel containing sodium dodecylsulfate

In order to study the fractional compositions of the histones isolated from the cotton plant, they have been subjected to electrophoretic separation in polyacrylamide gels (PAAGs) containing sodium dodecylsulfate (NA-DDS). It has been established that the rate and sequence of migration of the histones under consideration in PAAGs differ from the corresponding values of the histones of the calf thymus. A comparative evaluation of the histones of the cotton plant and of the calf thymus has been made by two-dimensional electrophoresis. The molecular weights of fractions of the cotton-plant histone were determined from their electrophoretic mobilities in PAAGs with Na-DDS:H1 - 23,500;H2A - 14,600; H2B - 14,960; H3 - 14,300; and H4 - 11,300.