Inactivation of extremely halophilic hide-damaging bacteria via low-level direct electric current |
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| Institution: | 1. Technical Education Faculty, Department of Electrical Education, Marmara University, Goztepe, Istanbul 34722, Turkey;2. Science and Letter Faculty, Department of Biology, Marmara University, Goztepe, ?stanbul 34722, Turkey;1. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;2. Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan;3. Research Center of Life Science and Biotechnology, Northeast Agricultural University, Harbin 150030, China;1. Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan;2. Center for Advanced Medicine and Clinical Research, Nagoya University Hospital, Nagoya 466-8550, Japan;3. Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan;4. Molecular Pharmacology and Pathology Program, Department of Pathology and Bosch Institute, The University of Sydney, Sydney, NSW 2006, Australia;1. Functional Nanomaterials and Polymer Nanocomposite Laboratory, Department of Physics, VIT-AP University, Amaravati, Guntur 522501, Andhra Pradesh, India;2. New Technologies – Research Center, University of West Bohemia, Plzeň 30100, Czech Republic;3. Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, NJ 07102, USA;1. Institute of Electronic Materials Technology, 133 Wolczynska st., 01-919 Warsaw, Poland;2. Industrial Research Institute for Automation and Measurements PIAP, Al. Jerozolimskie 202, 02-486 Warsaw, Poland;3. National Centre for Nuclear Research, A Soltana 7, 05-400 Otwock. Poland |
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| Abstract: | The presence of proteolytic and lipolytic extremely halophilic bacteria on brine-cured hides reduces the value of the hide as a raw material for leather manufacturing. Every brine curing raceway that produces hide contains a significant number of extremely halophilic bacteria that contaminate almost every hide. The usual brine raceway treatments used to control bacteria during curing do not control halophilic bacteria. The antibacterial effects of passing 0.5-A low level, direct electric current for 30 min on different species of protease, lipase, both protease and lipase producing extremely halophilic bacteria, as well as a mixed population of extremely halophilic bacteria, were examined in liquid media containing organic substances and 25% NaCl. Although the antibacterial effects varied with different extremely halophilic bacterial species, the application of current demonstrated an antibacterial effect within 20 min of treatment of all species tested. A mixed culture of extremely halophilic bacteria was killed within 10 min. It has been demonstrated that treatment time as short as 5 min is sufficient for the total inactivation of protease producing extremely halophilic bacteria of 107 CFU/mL while lipase or lipase and protease producing extremely halophilic bacteria were inactivated within 20 min. It was also found that the temperature and pH of the test medium increased during treatment. The maximum temperature rise was 9 °C, and the pH increased by 4. |
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