Reversal of myo-inositol metabolic level in the left dorsolateral prefrontal cortex of rats exposed to forced swimming test following desipramine treatment: an in vivo localized H-MRS study at 4.7 T |
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Authors: | Sang-Young Kim Chi-Bong Choi Hyun-Sung Lee Sung-Ho Lee Dong-Cheol Woo Hwi-Yool Kim Kwan-Soo Hong Chul-Hyun Lee Bo-Young Choe |
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Affiliation: | 1. Department of Biomedical Engineering, College of Medicine, The Catholic University of Korea, Seoul 137-040, South Korea;2. Research Institute of Biomedical Engineering, The Catholic University of Korea, Seoul 137-040, South Korea;3. Department of Radiology, Kyunghee University Medical Center, Hoekidong, Dongdaemungu, Seoul 130-702, South Korea;4. MRI Team, Korea Basic Science Institute, 804-1 Yangcheong-Ri, Ochang-Myun, Choongbuk, South Korea;5. Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 143-701, South Korea |
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Abstract: | The forced swimming test (FST) is a useful paradigm that is relatively quick and simple to perform and has been utilized to predict antidepressant activity based on learned helplessness as a model of depression. To date, few studies have used proton magnetic resonance spectroscopy (1H-MRS) to assess antidepressant effects in rats. The purpose of this study was to assess desipramine (DMI) effects on the left dorsolateral prefrontal cortex (DLPFC) of the rats, which were randomly assigned to three groups (control, n=10; FST+saline, n=10; FST+DMI, n=10), using single-voxel localization technique. All 1H-MRS experiments were performed on a Bruker 4.7-T scanner with 400 mm bore magnet, allowing for acquisition of in vivo 1H point-resolved spectroscopy spectra (TR/TE=3000/30 ms, number of data points=2048, NEX=512, voxel volume=27 μl, scan time=25 min). Proton metabolites were quantified automatically using LCModel software and were expressed as ratios to total creatine (Cr+PCr). Major target metabolites such as N-acetyl aspartate (NAA)+N-acetylaspartylglutamate (NAAG), glutamate+glutamine (Glu+Gln), glycerophosphorylcholine+phosphorylcholine (GPC+PCho), myo-inositol (mIns) and taurine (Tau) were successfully quantified with Cramer–Rao lower boundary ≤10%. There were significantly higher mIns/(Cr+PCr) and mIns/(NAA+NAAG) ratios in the FST+saline group compared to the control group. In the FST+DMI group, both mIns/(Cr+PCr) and mIns/(NAA+NAAG) ratios were significantly decreased to the level similar to those in the control group. No other metabolite ratios were significantly different among the three groups. Our findings suggest a possible role of altered mIns level within the left DLPFC of the rat model for depression. |
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Keywords: | Forced swimming test (FST) Proton magnetic resonance spectroscopy (1H-MRS) Left dorsolateral prefrontal cortex (DLPFC) Desipramine (DMI) Myo-inositol (mIns) |
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