Effect of inhibitors released during steam-explosion pretreatment of barley straw on enzymatic hydrolysis |
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Authors: | Ma Prado García-Aparicio Ignacio Ballesteros Alberto González José Miguel Oliva Mercedes Ballesteros Ma José Negro |
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Institution: | (1) Renewable Energies Department—CIEMAT, Avenida Complutense, 22 28040 Madrid, Spain |
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Abstract: | The influence of the liquid fraction (prehydrolysate) generated during steam-explosion pretreatment (210°C, 15 min) of barley
straw on the enzymatic hydrolysis was determined. Prehydrolysate was analyzed for degradation compounds and sugars' content
and used as a medium for enzymatic hydrolysis tests after pH adjusting to 4.8. Our results show that the presence of the compounds
contained in the prehydrolysate strongly affects the hydrolysis step (a 25% decrease in cellulose conversion compared with
control). Sugars are shown to be more potent inhibitors of enzymatic hydrolysis than degradation products. |
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Keywords: | Enzymatic hydrolysis barley straw inhibition steam explosion |
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