Quality Assessment of Veratrum nigrum L. by LC-ELSD Fingerprints and LC Quantitative Analysis |
| |
Authors: | Wang Lishu Zhao Daqing Liu Yonghong |
| |
Institution: | 1.School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, Shenyang, Liaoning, 110016, People’s Republic of China ; |
| |
Abstract: | A rapid and sensitive LC-MS method has been developed for the determination of luteolin-7-O-β-d-glucoside in rat plasma after solvent extraction. Separation was on an Elite Hypersil ODS2 column (250 mm × 4.6 mm i.d., 5 μm) with a mobile phase of acetonitrile-0.3% acetic acid (26:74, v/v). The samples were analyzed by using positive electrospray ionization MS in selected ion monitoring mode. The selected ions for luteolin-7-O-β-d-glucoside and the internal standard, isoquercitrin, were m/z 448.95 and m/z 464.95. Good linearity was observed over the range of 20–2,000 ng mL?1 with a lower limit of quantification of 20 ng mL?1. No interference peaks or matrix effects were observed. The validated method was applied to the pharmacokinetic study of luteolin-7-O-β-d-glucoside in rat plasma after intravenous administration of Kudiezi Injection. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|