Site-specific binding of chelerythrine and sanguinarine to single pyrimidine bulges in hairpin DNA |
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| Authors: | Li-Ping Bai Zongwei Cai Zhong-Zhen Zhao Kazuhiko Nakatani Zhi-Hong Jiang |
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| Institution: | (1) School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong;(2) Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Hong Kong;(3) Department of Regulatory Bioorganic Chemistry, The Institute of Scientific and Industrial Research (SANKEN), Osaka University, 8–1 Mihogaoka, Ibaraki 567–0047, Japan |
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| Abstract: | Spectrofluorometric titration, electrospray ionization time-of-flight mass spectrometric and UV melting methods were employed
to study the binding of chelerythrine and sanguinarine to bulged DNA. The results showed that both alkaloids bind specifically
to single pyrimidine (C, T) bulge sites. The ability of sanguinarine to bind to both regular and bulged hairpins was found
to be stronger than that of chelerythrine, but the binding selectivity of chelerythrine toward single-base bulges was much
larger than that of sanguinarine.
Figure Association constants for chelerythrine and sanguinarine toward regular and single-base bulged hairpins obtained from fluorometric
analysis |
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| Keywords: | Chelerythrine Sanguinarine Bulge DNA Fluorescence spectrometry ESI-MS |
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