The use of mobile phase pH and column temperature to reverse the retention order of enantiomers on chiral-AGP® |
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Authors: | A Karlsson A Aspegren |
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Institution: | 1. Analytical Chemistry, Astra H?ssle AB, 431 83, M?lndal, Sweden
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Abstract: | Summary Enantiomeric separation of mosapride and a structurally related compound was performed using chiral chromatography and experimental
design. Unique effects of mobile phase pH and column temperature made it possible to control the elution order of the enantiomers
when using Chiral-AGP as the solid phase. At a low mobile phase pH (<6) the (R)-enantiomer of mosapride elutes before the (S)-form whereas the (S)-enantiomer elutes first at a high mobile phase pH (>6). By using a mobile phase pH around 6, the column temperature could
also be used to control the elution order of the enantiomers of mosapride. Similar effects of mobile phase pH and column temperature
were obtained for the enantiomers of a structurally related compound, a metabolite (M1). Isocratic chromatographic systems
made it possible to determine enantiomeric impurities less than 0.1% in the respective enantiomer of mosapride. The enantiomers
of mosapride as well as the enantiomers of M1 could easily be separated simultaneously using Chiral-AGP and a simple gradient
elution.
Part of this work has been presented as lectures at HPLC'96 in San Francisco USA, at AAPS-96 in Seattle USA and as a poster
at HPLC'95 in Innsbruck Austria. |
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Keywords: | Column liquid chromatography Experimental design Mosapride Reversal of retention order Effect of pH and column temperature |
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