The influence of complexing pharmaceutical compositions on alkaline phosphatase |
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| Authors: | L. F. Atyaksheva E. S. Chukhrai N. D. Stepina N. N. Novikova E. A. Yur’eva |
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| Affiliation: | (1) Department of Nutrition and Food Hygiene, School of Public Health, China Medical University, No. 92 Beier Road, Heping District, Shenyang, Liaoning, Postal code: 110001, People’s Republic of China;(2) Department of Physiology, School of Basic Medicine, China Medical University, Heping District, Shenyang, Liaoning, People’s Republic of China;(3) Department of Environmental and Occupational Health, School of Public Health, China Medical University, Heping District, Shenyang, Liaoning, People’s Republic of China;(4) Seven-year class of Clinical medicine, China Medical University, Heping District, Shenyang, Liaoning, People’s Republic of China; |
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| Abstract: | It is established that the pharmaceutical compositions xydiphon, medifon, succimer, and EDTA, which are used as complexing agents for accelerating the excretion of heavy metals from human organism, at certain concentrations inhibit enzyme alkaline phosphatase (AP). It is concluded that xydiphon and EDTA have a noticeable effect on AP activity at concentrations over 0.01 mM; medifon and succimer, at concentrations of over 0.3–0.5 mM. The enzyme’s inhibition constants and type of inhibition are determined. Xydiphon is found to manifest the highest affinity to AP (K I = 0.35 mM). It is shown by kinetic analysis that dissociative chemoinactivation of the enzyme takes place under the action of complexing agents. The corresponding kinetic parameters are calculated. |
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