Stability of anionic liposome-cationic polymer complexes in water-salt media |
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Authors: | A A Yaroslavov A A Efimova A V Sybachin V A Izumrudov V V Samoshin I I Potemkin |
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Institution: | 1.Department of Chemistry,Moscow State University,Moscow,Russia;2.Department of Physics,Moscow State University,Moscow,Russia;3.University of the Pacific,Stockton,USA |
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Abstract: | Laser microelectrophoresis, dynamic light scattering, and fluorescence and UV spectroscopy are employed to study poly-N-ethyl-4-vinylpyridinium bromide adsorption on the surface of bilayer lipid vesicles (liposomes) formed from mixtures of anionic
phosphatidyl serine and electroneutral phosphatidylcholine. It is established that polycation adsorption is accompanied by
the neutralization of charges on liposomes and their aggregation. The subsequent addition of a low-molecular-weight salt (NaCl)
solution to suspensions of complexes causes them to dissociate into their initial components, while the stability of the complexes
with respect to the salt action increases with the fraction of the anionic lipid in the liposome membranes. The data obtained
are interpreted from the position of the formation-disintegration of a molecular capacitor, the charge of which is generated
by spatially separated anionic lipids located in the bilayer membrane and cationic units of the adsorbed polyamine. |
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