Gender determination of fertilized unincubated chicken eggs by infrared spectroscopic imaging |
| |
Authors: | Gerald Steiner Thomas Bartels Allison Stelling Maria-Elisabeth Krautwald-Junghanns Herbert Fuhrmann Valdas Sablinskas Edmund Koch |
| |
Institution: | (1) Faculty of Medicine Carl Gustav Carus, Clinical Sensoring and Monitoring, Dresden University of Technology, Fetscherstr. 74, 01307 Dresden, Germany;(2) Faculty of Veterinary Medicine, Clinic for Birds and Reptiles, University of Leipzig, An den Tierkliniken 17, 04103 Leipzig, Germany;(3) Faculty of Veterinary Medicine, Institute of Physiological Chemistry, University of Leipzig, An den Tierkliniken 1, 04103 Leipzig, Germany;(4) Faculty of Physics, Department of General Physics and Spectroscopy, University of Vilnius, 01108 Vilnius, Lithuania |
| |
Abstract: | Each year, billions of day-old layer chicks are produced in the world. Since only female chicks are reared for egg production,
the chicks must be sexed and the unwanted male layer chicks are culled. The culling of male chicks is a serious problem, both
in terms of animal welfare and waste disposal. The germinal disc in fertilized but unincubated eggs contains already several
thousands of blastoderm cells. The cellular DNA in birds is different for male and female chicks. The difference in DNA content
between male and female chicks is around 2% and is measurable by Fourier transform infrared (FT-IR) spectroscopy. In this
study, small amounts of blastoderm cells from 22 chicken eggs were characterized by attenuated total reflection FT-IR spectroscopic
imaging and classified by linear discriminant analysis. Polymerase chain reaction (PCR) was used as a reference method to
determine the gender. The spectroscopic results demonstrate that male blastoderm cells exhibit a higher content of DNA than
cells from female blastoderm. The spectroscopic-based gender determination led to the same result as the PCR analysis. FT-IR
spectroscopic imaging allows the gender determination of unincubated eggs within a few seconds based on the accurate determination
of the different DNA contents in blastoderm cells of both sexes. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|