Liposomes Prepared from Inverse Micelles

A method for the preparation of liposome is introduced, which contains two experimental steps: (a) inverse micelles of lecithin are formed in water-in-oil system by sonication; (b) the micelles are spread on the water surface, passed Through the oil-water interface, and transformed into liposomes in the water phase. The main advantage of this method is that the inner aqueous solution encapsulated by liposomes could be different from their enviromental medium. The liposome size is less than 0.5 μm in diameter by atomic force microscope. Comparison of activities of urease with and without liposome encapsulation suggested that urease was well entraped into liposomes.