Tb3+ as Fluorescent Probe in the Study of the Interaction of Other Lanthanide Ions with Trichosanthin |
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Authors: | HAO Qiang LI Wen-lian ZHANG Yan YU Gui YANG Hong ZHAO Xu YAO Qi-Zhi LI Qing-shan |
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Institution: | 1. Department of Molecular Biofogy and National Laboratory of enzyme engineering, Jiln University, Changchun, 130023;
2. Changchun Institute of Physics, Academia Sinica, Changchun, 130023;
3. Graduate School of Acadrmia, Beijing, 100039 |
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Abstract: | The interaction of lanthanide (Ln3+)ions with trchosanthin(TCS)was investi-gated using Tb3+ as fluorescent probe.The metal-binding sites of the protein were probed by means of adding the Tb3+ into the protein solutions.If other Ln3+ ions exist in the Tb3+ protein complex system, they would compete against Tb3+ in the binding sites of the protein, and so the fluorescence intensity of Tb3+ decreased,which is called quenching effect.The quencliing effect is related to the ionic radii of the Ln3+ ions and energy transfer from Tb3+ protein to Ln3+ ions.Based on the ex-perimental results,a schematic of the energy transfer from protein-Tb3+ complex to protein-Ln3+ complex is suggested. |
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Keywords: | Trichosanthin Energy transfer Protein-Ln3+ complex |
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