| 磺化竹红菌素对蛋白质荧光猝灭机理的研究 |
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| 引用本文： | 乐嘉昌,杜长征,庞素珍,安静仪,蒋丽金.磺化竹红菌素对蛋白质荧光猝灭机理的研究[J].影像科学与光化学,1994,12(3):221-228. |
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| 作者姓名： | 乐嘉昌 杜长征 庞素珍 安静仪 蒋丽金 |
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| 作者单位： | 1. 中国科学院生物物理研究所, 北京100101;2. 中国科学院感光化学研究所, 北京100101 |
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| 基金项目： | 中国科学院感光化学研究所光化学开放实验室资助 |
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| 摘 要： | 本文详细研究了磺化竹红菌素对多种蛋白质在溶液状态下的荧光猝灭过程。结果表明,磺化竹红菌素对多种蛋白质荧光猝灭服从Stern-Volmer曲线,实验观察了温度、粘度、pH值和盐酸胍对荧光猝灭过程的影响。由于磺化竹红菌素是一两性分子,对于不同蛋白具有不同猝灭过程;磺化竹红菌素对蛋白质的荧光猝灭常数Kq在1013mol/L·s-1左右,这说明,磺化竹红菌素是一种比其它蛋白质荧光猝灭剂更加有效的荧光猝灭剂。
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| 关 键 词： | 荧光猝灭 蛋白质 竹红菌素 |
| 收稿时间： | 1993-06-25 |
STUDIES OF QUENCHING OF PROTEIN FLUORESCENCE BY SULPHATED HYPOCRELLIN |
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| YUE JIA-CHANG,DU CHANG-ZHENG,PANG SU-ZHEN,AN JING-YI,JIANG LI-JIN.STUDIES OF QUENCHING OF PROTEIN FLUORESCENCE BY SULPHATED HYPOCRELLIN[J].Imaging Science and Photochemistry,1994,12(3):221-228. |
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| Authors: | YUE JIA-CHANG DU CHANG-ZHENG PANG SU-ZHEN AN JING-YI JIANG LI-JIN |
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| Institution: | 1. Institue of Biophysics, Academia Sinicca, Beijing 100101, P.R. China;2. Institute of Photographic Chemistry, Academia Sinica, Beijing 100101, P.R. China |
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| Abstract: | This papre studies quenching of proteins fluorescence by sulphated Hypocrellin(HA-SO3).The experimental results show that the quenching of protein fluorescence by sul-phated Hypocrellin follows the classical Stern-Volmer equation. The variations of other conditions such as temperatures,viscosities,pH,guanidine-HCl ionic strength were also investigated.The sulphated Hypocrellin is an amphibious molecule with hydrophobic and hydrophilic.The quenching of various proteins may be at different mechanism;The quench fluorescence of protein tryphanyl residues is relatively large rate constant(the Kq values rang from 1-2 × 1013mol/L·s-1).All results also show that HA-SO3 is a more efficientquencher than others. |
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| Keywords: | fluorescence quenching protein hypocrellin |
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