| 红藻条斑紫菜R-藻红蛋白中的能量传递过程 |
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| 引用本文： | 伍华菊,张建平,夏安东,朱晋昌,蒋丽金. 红藻条斑紫菜R-藻红蛋白中的能量传递过程[J]. 影像科学与光化学, 1995, 13(1): 53-60. DOI: 10.7517/j.issn.1674-0475.1995.01.53 |
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| 作者姓名： | 伍华菊 张建平 夏安东 朱晋昌 蒋丽金 |
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| 作者单位： | 1. 中国科学院感光化学研究所, 北京100101;2. 合肥中国科学技术大学国家同步辐射实验室 |
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| 基金项目： | “八五”重大基础研究项目;国家自然科学基金 |
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| 摘 要： | 本文通过对条斑紫菜R-PE(藻红蛋白)及其α-β-γ亚基的吸收光谱和荧光光谱进行计算机解叠,研究了R-PE内发色团之间的能量传递过程,并对R-PE及亚基内的各发色团进行了“s”和“f”型的指认。发现在亚基中为“f”型的发色团在R-pE(αβ)6γ中起着“s”型发色团的作用,且将能量传递给最后的“f”型发色团。荧光激发偏振光谱进一步证明了R-PE内的能量转移过程与计算机解叠的结果一致。
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| 关 键 词： | 光谱解叠 能量传递 藻胆蛋白 |
| 收稿时间： | 1994-03-23 |
ENERGY TRANSFER IN R-PHYCOERYTHRIN FROM RED ALGA PROPHYRA YEZOENSIS |
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| WU HUA-JU,ZHANG JIAN-PING,XIA AN-DONG,ZHU JING-CHANG,JIANG LI-JIN. ENERGY TRANSFER IN R-PHYCOERYTHRIN FROM RED ALGA PROPHYRA YEZOENSIS[J]. Imaging Science and Photochemistry, 1995, 13(1): 53-60. DOI: 10.7517/j.issn.1674-0475.1995.01.53 |
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| Authors: | WU HUA-JU ZHANG JIAN-PING XIA AN-DONG ZHU JING-CHANG JIANG LI-JIN |
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| Affiliation: | 1. Institute of Photographic Chemistry, The chinese Academy of Sciences, Beijing 100101, P.R. China |
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| Abstract: | The energy transfer processes ih R-phycoerythrin as well as its α,β,γ,subunits isolated from red alga prophyra yezoensis were studied by absorption, fluorescence spectra and the deconvolution of these spectra.A tentative assignment of“s”and“f”type chromophores in R-PE and α,β,γ subunits was also maken, The“f”type chromophore in β(or γ)subunitis found to be an s-type chromophore in R-PE[(αβ)6γ], and transfer its energy to the final“f”type chromophores. The results obtained from the fluorescence excitation polarization spectrum of R-PE are in good agreement with the deconvolution results for the energy transfer processes. |
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| Keywords: | deconvolution energy transfer phycobiliprotein |
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