Rhenium and technetium tricarbonyl complexes anchored by 5-HT1A receptor-binding ligands containing P,O/N donor atom sets |
| |
Authors: | Elisa Palma Ângela Domingos Roger Alberto |
| |
Institution: | a Departamento de Química, ITN, Estrada Nacional 10, 2686-953 Sacavém Codex, Portugal b Institute of Inorganic Chemistry, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland c Institute of Bioinorganic & Radiopharmaceutical Chemistry, Forschungszentrum Rossendorf, D-01314 Dresden, Germany |
| |
Abstract: | The (2-methoxyphenyl)piperazine pharmacophore, a part of the WAY 100635 structure, has been functionalized with phosphinoarylbenzylamide or phosphinoarylbenzylamine chelator groups using propylene or hexylene alkyl chains as linkers (L2-L4). These heterofunctionalized phosphines bearing an arylpiperazine moiety have been used to stabilize rhenium tricarbonyl complexes of the type Re(CO)3Br(κ2-L)] (4, L = L2; 5, L = L3; 6, L = L4), which have been fully characterized, including by X-ray crystallographic analysis in the case of compounds 4 and 5. These monomeric complexes are six-coordinate, displaying a distorted octahedral coordination geometry with a facial arrangement of the carbonyl groups. The other three remaining positions are occupied by a bromide and by the bidentate heterofunctionalized phosphine, which coordinates through the phosphorus and the oxygen atom or through the phosphorus and the nitrogen atom in 4 and 5, respectively. The 99mTc complexes (3a-6a) were also prepared and their characterization established by comparative HPLC, using the Re complexes as surrogates. The in vitro binding affinity for the 5HT1A receptor subtype and the selectivity against the 5HT2A receptors for the rhenium complexes were determined. Compound 3 is the only one which presents a reasonable affinity and selectively towards 5HT1A (IC50 = 20 nM) and 5HT2A (IC50 = 4680 nM) receptors, respectively. When the spacer length between the chelate unit and receptor binding domain increased and/or the amide group in the chelator was replaced by a secondary amine unacceptable affinity values for 5HT1A receptors (IC50 = 200-1100 nM) and lost of selectivity were observed. |
| |
Keywords: | Rhenium Technetium Phosphine Radiopharmaceuticals 5HT1A receptor-ligands |
本文献已被 ScienceDirect 等数据库收录! |
|