Simultaneous determination of residual antiparasitic lactones in bovine muscle and liver by liquid chromatography with fluorescence detection |
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Authors: | Nagata Tomoko Miyamoto Fumio Hasegawa Yasuyuki Ashizawa Eiichi |
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Affiliation: | The Public Health Laboratory of Chiba Prefecture, 666-2, Nitona-cho, Chuo-ku, Chiba City 260-7815, Japan. t.ngt2@mc.pref.chiba.jp |
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Abstract: | Abamectin, doramectin, eprinomectin, ivermectin, milbemectin, and moxidectin in bovine muscle and liver were extracted with acetonitrile. The extracts were partitioned with n-hexane and then evaporated to dryness. The residue was cleaned up on Bond Elute NH2 cartridge, and the drugs were eluted from the cartridge with methanol-ethyl acetate (3 + 7). The eluate was evaporated to dryness, and residues were derivatized with N,N-dimethylformamide-acetic anhyride-1-methylimidazole. The derivatives were determined by liquid chromatography with fluorescence detection. Recoveries of the 6 drugs were 79.6-63.8% in muscle and 71.6-60.6% in liver at 0.01 ppm levels. The quantitation limits were 5 ppb for each drug. |
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