Ambient Molecular Analysis of Biological Tissue Using Low-Energy,Femtosecond Laser Vaporization and Nanospray Postionization Mass Spectrometry |
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| Authors: | Fengjian Shi,Paul M. Flanigan Suffix" >IV,Jieutonne J. Archer,Robert J. Levis |
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| Affiliation: | 1.Department of Chemistry,Temple University,Philadelphia,USA;2.Center for Advanced Photonics Research,Temple University,Philadelphia,USA;3.Signature Science, LLC.,Egg Harbor Township,USA |
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| Abstract: | Direct analysis of plant and animal tissue samples by laser electrospray mass spectrometry (LEMS) was investigated using low-energy, femtosecond duration laser vaporization at wavelengths of 800 and 1042 nm followed by nanospray postionization. Low-energy (<50 μJ), fiber-based 1042 nm LEMS (F-LEMS) allowed interrogation of the molecular species in fresh flower petal and leaf samples using 435 fs, 10 Hz bursts of 20 pulses from a Ytterbium-doped fiber laser and revealed comparable results to high energy (75–1120 μJ), 45 fs, 800 nm Ti:Sapphire-based LEMS (Ti:Sapphire-LEMS) measurements. Anthocyanins, sugars, and other metabolites were successfully detected and revealed the anticipated metabolite profile for the petal and leaf samples. Phospholipids, especially phosphatidylcholine, were identified from a fresh mouse brain section sample using Ti:Sapphire-LEMS without the application of matrix. These lipid features were suppressed in both the fiber-based and Ti:Sapphire-based LEMS measurements when the brain sample was prepared using the optimal cutting temperature compounds that are commonly used in animal tissue cryosections. |
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