Amperometric methylene blue-mediated sensor highly sensitive to hydrogen peroxide based on a composite membrane of regenerated silk fibroin and poly-vinyl alcohol as immobilization matrix for horseradish peroxidase |
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| Authors: | Haiying Liu Jianghong Qian Yongcheng Liu Tongyin Yu Jiagi Deng and Deyao Qi |
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| Institution: | (1) Department of Chemistry and Chemical Engineering, Shanghai University, 200 072 Shanghai, People’s Republic of China;(2) Department of Chemistry, Fudan University, 200 433 Shanghai, People’s Republic of China;(3) Department of Macromolecular Science, Fudan University, 200 433 Shanghai, People’s Republic of China |
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| Abstract: | Horseradish peroxidase (HRP) was effectively entrapped in a novel composite membrane of poly-vinyl alcohol and regenerated
silk fibroin, and IR was employed to provide a useful insight into the structure of the composite membrane. A methylene bluemediated
sensor highly sensitive to hydrogen peroxide was constructed, which was based on the immobilization of HRP in the composite
membrane. Cyclic voltammetry and amperometric measurement were utilized to demonstrate the feasibility of electron communication
between immobilized HRP and a glassy carbon electrode in the bioelectrocatalytic reduction of hydrogen peroxide via methylene
blue. Performance and characteristics of the sensor were evaluated with regard to response time, detection limit, selectivity,
and dependence on temperature and pH as well as operating and storage stability. The sensor possesses a variety of characteristics
including high sensitivity, rapid response time and a low detection limit of 0.1 μmol/L. |
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