Fluorimetric detection of pathogenic bacteria using magnetic carbon dots |
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Authors: | Mukesh Lavkush Bhaisare Gangaraju Gedda M Shahnawaz Khan Hui-Fen Wu |
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Institution: | 1. Department of Chemistry and Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung, 80424, Taiwan;2. School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan;3. Institute of Medical Science and Technology, National Sun Yat-Sen University, 80424, Taiwan;4. Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University and Academia Sinica, Kaohsiung, 80424, Taiwan |
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Abstract: | A novel and facile approach of pathogenic bacteria detection, which utilizes fluorescent sensing and bacteria capture with Magnetic carbon dots (Mag-CDs), was proposed in this work. Magnetic nanoparticles were synthesized and then decorated with C-dots, and further functionalized with amine groups (chitosan). In this way, bacteria were strongly anchored on the hybrid material Mag-CDs for highly sensitive fluorescent detection. The Mag-CDs were characterized by UV–vis, FT-IR spectra, TEM images, XRD, and EDX. The characterizations validate the fabrication of amine-Mag-CDs and the promising applications of this material. Fluorescence spectroscope and MALDI-MS were used for the detection and identification of bacterial strains, respectively. The limit of detection for Staphylococcus aureus and Escherichia coli was found to be 3 × 102 and 3.5 × 102 cfu mL−1, respectively. With these encouraging results, it is expected that it would open revenues for promising applications of Mag-CDs nanomaterial. |
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Keywords: | Magnetic carbon dots Staphylococcus aureus Escherichia coli Fluorescence detection MALDI-MS |
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