An ultra-sensitive and colorimetric sensor for copper and iron based on glutathione-functionalized gold nanoclusters |
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Authors: | Qian Zhao Huan Yan Ping Liu Yingyi Yao Yudong Wu Jian Zhang Hengxuan Li Xiaoqun Gong Jin Chang |
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Institution: | 1. School of Life Sciences, Tianjin University, Tianjin Engineering Center of Micro-Nano Biomaterials and Detection-Treatment Technology (Tianjin), 92 Weijin Road, Nankai District, Tianjin 300072, China;2. Medical Laboratory, Tianjin Medical University General Hospital, Tianjin, 300052, China;3. Bioscience(Tianjin) Diagnostic Technology CO., LTD, Tianjin, 300300, China |
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Abstract: | Here, we report an ultra-sensitive and colorimetric sensor for the detection of Fe3+ or Cu2+ successively using glutathione-functionalized Au nanoclusters (GSH-AuNCs). For GSH-AuNCs can catalytically oxidize peroxidase substrates, such as 3, 3′, 5, 5′-tetramethylbenzidine (TMB), colored products are formed in the presence of H2O2. While upon the addition of Fe3+ or Cu2+ into the GSH-AuNCs-TMB-H2O2 system, diverse color and absorbance of the system was obtained due to the self oxidation of Fe3+ and the inhibition of peroxidase-like activity of GSH-AuNCs. With the introduction of ethylene diamine tetraacetic acid (EDTA) or ammonium fluoride (NH4F) to GSH-AuNCs-TMB-H2O2+Cu2+ system or GSH-AuNCs-TMB-H2O2+Fe3+ system respectively, a restoration of color and absorbance of system was realized. On the basis of above phenomenon, a colorimetric and quantitative approach for detecting Fe3+ and Cu2+ was developed with detection limit of 1.25 × 10−9 M and 1.25 × 10−10 M respectively. Moreover, the concentration of Fe3+ and Cu2+ in human serums was also accurate quantified by this method. So this design strategy realized the simple and simultaneous detection of Fe3+ and Cu2+, suggesting significant potential in clinical diagnosis. |
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Keywords: | Au nanoclusters Peroxidase-like activity Fe3+ Cu2+ TMB |
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