A quick response fluorescent probe based on coumarin and quinone for glutathione and its application in living cells |
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| Authors: | Xi Dai Zhi-Fang Du Li-Hong Wang Jun-Ying Miao Bao-Xiang Zhao |
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| Affiliation: | 1. Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China;2. Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, PR China;3. Taishan College, Shandong University, Jinan 250100, PR China |
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| Abstract: | We have designed and synthesized a simple but effective fluorescent probe for sensing glutathione (GSH) by PET process based on coumarin and quinone, which worked as fluorophore and reaction site, respectively. The probe could discriminate GSH from cysteine and homocysteine within 1 min in PBS-buffered solution. The sensing mechanism was confirmed by density functional theory (DFT), viscosity test, fluorescence spectrum analysis and HRMS, respectively. The probe has a low limit of detection (0.1 μM) and finally been used in cell imaging successfully. |
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| Keywords: | Coumarin Quinone Glutathione Fluorescent probe Living cells |
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