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A novel reagentless glutamate microband biosensor for real-time cell toxicity monitoring |
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| Authors: | G. Hughes R.M. Pemberton P.R. Fielden J.P. Hart |
| Affiliation: | 1. Centre for Research in Biosciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, Coldharbour Lane, Bristol, BS16 1QY, United Kingdom;2. Department of Chemistry, Lancaster University, Bailrigg, Lancaster, LA1 4YB, United Kingdom |
| Abstract: | A reagentless glutamate biosensor was applied to the determination of glutamate released from liver hepatocellular carcinoma cells (HepG2) in response to toxic challenge from various concentrations of paracetamol. A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola's Blue (MB-SPCE) served as the electron mediator for the oxidation of NADH. |
| Keywords: | Glutamate Microband Biosensor Cell toxicity Amperometric HepG2 |
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