Titania nanotubes decorated with gold nanoparticles for electrochemiluminescent biosensing of glycosylated hemoglobin |
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Authors: | Qun Zhao Shan Tang Chen Fang Yi-Feng Tu |
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Institution: | 1. Institute of Analytical Chemistry, Dushu Lake Campus, Soochow University, Industrial Park, Suzhou, 215123, PR China;2. College of Chemistry and Chemical Engineering, Nantong University, Nantong, 226019, PR China;3. Second Affiliated Hospital, Soochow University, Suzhou, 215004, PR China |
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Abstract: | A glycated hemoglobin (HbA1c) biosensor with high performance has been constructed in this work. Here the fructosyl amino acid oxidase was immobilized onto a pre-functionalized indium tin oxide glass with titania nanotubes decorated with gold nanoparticles. The property of nanocomposite was characterized by transmission electromicroscopy, scanning electron microscopy, electrochemistry and spectroscopy. Under the optimum conditions, fructosyl valine was detected by this biosensor. It exhibited a linear detection range from 4.0 × 10−9 M to 7.2 × 10−7 M, and a limit of detection for 3.8 × 10−9 M at the signal-to-noise ratio of 3. Thus the HbA1c level in whole blood samples of healthy individuals or diabetic patients were evaluated with designed biosensor after pre-treatment of hydrolysis. The results of our detection were closely consistent with that of the standard method. At the same time, our biosensor has some advantages including high sensitivity, disposable usage and low cost, which implies its great promising application in point-of-care testing of HbA1c. |
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Keywords: | Electrochemiluminescence Luminol AuNPs/TiNTs Glycosylated hemoglobin Fructosyl amino-acid oxidase |
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