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Upconverting nanophosphors as reporters in a highly sensitive heterogeneous immunoassay for cardiac troponin I
Authors:Nina Sirkka,Annika Lyytikä  inenTanja Savukoski,Tero Soukka
Affiliation:Department of Biochemistry/Biotechnology, University of Turku, Tykistökatu 6 A, 20520 Turku, Finland
Abstract:Photon upconverting nanophosphors (UCNPs) have a unique capability to produce anti-Stokes emission at visible wavelengths via sequential multiphoton absorption upon infrared excitation. Since the anti-Stokes emission can be easily spectrally resolved from the Stokes' shifted autofluorescence, the upconversion luminescence (UCL) is a highly attractive reporter technology for optical biosensors and biomolecular binding assays – potentially enabling unprecedented sensitivity in separation-based solid-phase immunoassays.
Keywords:Cardiac troponin I   Immunoassay   Upconversion luminescence   Upconverting nanophosphors
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