Inkjet-assisted layer-by-layer printing of quantum dot/enzyme microarrays for highly sensitive detection of organophosphorous pesticides |
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| Authors: | Enxiao Luan Zhaozhu Zheng Xinyu Li Hongxi Gu Shaoqin Liu |
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| Affiliation: | 1. State Key Laboratory of Urban Water Resource and Environment, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China;2. Micro- and Nanotechnology Research Center, Harbin Institute of Technology, Harbin 150080, China |
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| Abstract: | We present a facile fabrication of layer-by-layer (LbL) microarrays of quantum dots (QDs) and acetylcholinesterase enzyme (AChE). The resulting arrays had several unique properties, such as low cost, high integration and excellent flexibility and time–saving. The presence of organophosphorous pesticides (OPs) can inhibit the AChE activity and thus changes the fluorescent intensity of QDs/AChE microscopic dot arrays. Therefore, the QDs/AChE microscopic dot arrays were used for the sensitive visual detection of OPs. Linear calibration for parathion and paraoxon was obtained in the range of 5–100 μg L−1 under the optimized conditions with the limit of detection (LOD) of 10 μg L−1. The arrays have been successfully used for detection of OPs in fruits and water real samples. The new array was validated by comparison with conventional high performance liquid chromatography-mass spectrometry (HPLC-MS). |
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| Keywords: | Quantum dot Acetylcholinesterase Biosensor Organophosphorus pesticide Layer-by-layer assembly Inkjet-printing |
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