Quantitative bioimaging of platinum group elements in tumor spheroids |
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| Authors: | Ann-Christin Niehoff,Jonas Grü nebaum,Aline Moosmann,Dennis Mulac,Judith Sö bbing,Rebecca Niehaus,Rebecca Buchholz,Sabrina Krö ger,Arno Wiehe,Sylvia Wagner,Michael Sperling,Hagen von Briesen,Klaus Langer,Uwe Karst |
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| Affiliation: | 1. Institute of Inorganic and Analytical Chemistry, University of Münster, Corrensstr. 30, 48149 Münster, Germany;2. Department of Pharmaceutical Technology and Biopharmacy, University of Münster, Corrensstr. 48, 48149 Münster, Germany;3. Department of Bioprocessing & Bioanalytics, Fraunhofer Institute for Biomedical Engineering, Joseph-von-Fraunhofer-Weg 1, 66280 Sulzbach, Germany;4. Biolitec Research GmbH, Otto-Schott-Str. 15, 07745 Jena, Germany |
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| Abstract: | Limited drug penetration into tumor tissue is a significant factor to the effectiveness of cancer therapy. Tumor spheroids, a 3D cell culture model system, can be used to study drug penetration for pharmaceutical development. In this study, a method for quantitative bioimaging of platinum group elements by laser ablation (LA) coupled to inductively coupled plasma mass spectrometry (ICP-MS) is presented. Different matrix-matched standards were used to develop a quantitative LA-ICP-MS method with high spatial resolution. To investigate drug penetration, tumor spheroids were incubated with platinum complexes (Pt(II)acetylacetonate, cisplatin) and the palladium tagged photosensitizer 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP). Distribution and accumulation of the pharmaceuticals were determined with the developed method. |
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| Keywords: | LA-ICP-MS Quantification Tumor spheroids Pt cytostatics Photosensitizer |
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