A simple,fast and low-cost turn-on fluorescence method for dopamine detection using in situ reaction |
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| Authors: | Xiulan Zhang Yonggang Zhu Xie Li Xuhong Guo Bo Zhang Xin Jia Bin Dai |
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| Affiliation: | 1. School of Chemistry and Chemical Engineering/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region, Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Shihezi, 832003, People''s Republic of China;2. Microfluidics and Fluid Dynamics Laboratory, CSIRO Manufacturing, Private Bag 10, Clayton South, Victoria, 3168, Australia;3. State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai, 200237, People''s Republic of China;4. Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi, 832000, People''s Republic of China |
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| Abstract: | A simple, fast and low-cost method for dopamine (DA) detection based on turn-on fluorescence using resorcinol is developed. The rapid reaction between resorcinol and DA allows the detection to be performed within 5 min, and the reaction product (azamonardine) with high quantum yield generates strong fluorescence signal for sensitive optical detection. The detection exhibits a high sensitivity to DA with a wide linear range of 10 nM–20 μM and the limit of detection is estimated to be 1.8 nM (S/N = 3). This approach has been successfully applied to determine DA concentrations in human urine samples with satisfactory quantitative recovery of 97.84%–103.50%, which shows great potential in clinical diagnosis. |
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| Keywords: | Resorcinol Dopamine detection Azamonardine Turn-on fluorescence |
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