Chitosan-iron oxide nanocomposite based electrochemical aptasensor for determination of malathion |
| |
Authors: | Nirmal Prabhakar Himkusha ThakurAnu Bharti Navpreet Kaur |
| |
Institution: | Department of Biochemistry, Panjab University, Chandigarh 160014, India |
| |
Abstract: | An electrochemical aptasensor based on chitosan-iron oxide nanocomposite (CHIT-IO) film deposited on fluorine tin Oxide (FTO) was developed for the detection of malathion. Iron oxide nanoparticles were prepared by co-precipitation method and characterized by Transmission electron microscopy and UV–Visible spectroscopy. The biotinylated DNA aptamer sequence specific to the malathion was immobilized onto the iron oxide doped-chitosan/FTO electrode by using streptavidin as linking molecule. Various characterization studies like Field Emission-Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR), and Electrochemical studies were performed to attest the successful fabrication of bioelectrodes. Experimental parameters like aptamer concentration, response time, stability of electrode and reusability studies were optimized. Aptamer immobilized chitosan-iron oxide nanocomposite (APT/SA/CHIT-IO/FTO) bioelectrodes exhibited LOD of about 0.001 ng/mL within 15 min and spike-in studies revealed about 80–92% recovery of malathion from the lettuce leaves and soil sample. |
| |
Keywords: | Electrochemical biosensor Chitosan-iron oxide nanocomposite Aptamer Malathion Organophosphates |
本文献已被 ScienceDirect 等数据库收录! |