Colorimetric detection of urea,urease, and urease inhibitor based on the peroxidase-like activity of gold nanoparticles |
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| Authors: | Hao-Hua Deng Guo-Lin Hong Feng-Lin Lin Ai-Lin Liu Xing-Hua Xia Wei Chen |
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| Institution: | 1. Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou, 350004, China;2. Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Fujian Medical Univeristy, Fuzhou, 350004, China;3. Department of Laboratory Medicine, The First Affiliated Hospital of Xiamen University, Xiamen, 361003, China;4. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China |
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| Abstract: | Herein, we reported for the first time that gold nanoparticles-catalyzed 3,3′,5,5′-tetramethylbenzidine-H2O2 system can serve as an ultrasensitive colorimetric pH indicator. Gold nanoparticles acted as a catalyst and imitated the function of horseradish peroxidase. The absorbance at 450 nm of the yellow-color product in the catalytic reaction exhibited a linear fashion over the pH range of 6.40–6.60. On the basis of this property, we constructed a novel sensing platform for the determination of urea, urease, and urease inhibitor. The limit of detection for urea and urease was 5 μM and 1.8 U/L, respectively. The half-maximal inhibition value IC50 of acetohydroxamic acid was found to be 0.05 mM. Urea in human urine and urease in soil were detected with satisfied results. |
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| Keywords: | Gold nanoparticles Peroxidase-like activity Urea Urease Acetohydroxamic acid |
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