Application of DNA aptamers as sensing layers for detection of carbofuran by electrogenerated chemiluminescence energy transfer |
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Authors: | Shuhuai Li Xuejin Wu Chunhua Liu Guihao Yin Jinhui Luo Zhi Xu |
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Affiliation: | 1. Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China;2. Laboratory of Quality & Safety Risk Assessment for Tropical Products (Haikou) Ministry of Agriculture, Haikou, 571101, China |
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Abstract: | In this study, an electrogenerated chemiluminescence (ECL) sensing platform for carbofuran detection was constructed based on ECL energy transfer (ECRET) and carbon dot (C-dot)-tagged aptamers as the recognition element. Fullerene (C60)-loaded gold nanoparticles (C60-Au) were used as the energy donor, modified on a glassy carbon electrode. C-dot-tagged DNA aptamers were used as the receptor, and ECRET then occurred between C60-Au and C-dots. After accepting the energy, the C-dots acted as a signal indicator and showed decreased signal intensity in the presence of targets, which competitively bound to DNA aptamers and blocked energy transfer. Using this robust, straight-forward strategy, the sensor showed a linear ECL response to carbofuran at concentrations from 2.0 × 10−11 mol L−1 to 8.0 × 10−9 mol L−1. The detection limit of this assay was shown to be 8.8 × 10−13 mol L−1. Thus, the sensing approach described in this study could be adapted for use in the detection of various pesticide residue targets. |
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Keywords: | Aptasensor Fullerene Electrogenerated chemiluminescence Gold nanoparticles Carbofuran |
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