| 用L-脯氨酸衍生物作为手性流动相添加剂拆分对映异构体 |
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| 引用本文： | 常银霞[1,2] 候志林 黎其万 高天荣 袁黎明. 用L-脯氨酸衍生物作为手性流动相添加剂拆分对映异构体[J]. 分析化学, 2006, 34(9): 100-104 |
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| 作者姓名： | 常银霞[1 2] 候志林 黎其万 高天荣 袁黎明 |
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| 作者单位： | [1]云南师范大学化学化工学院,昆明650092 [2]山西师范大学化学与材料科学学院,临汾041000 [3]云南省农业科学院质量标准与检测技术研究所,昆明650223 |
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| 基金项目： | 本文系国家自然科学基金（No.30160092）、高等学校优秀青年教师教学科研奖励计划和云南省自然科学基金（No.2004B0013R）资助项目 |
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| 摘 要： | 用自制的N-十二酰基-L-脯氨酸和N-十二酰基-L-脯氨酸-3，5-二甲基苯胺两种L-脯氨酸衍生物作手性流动相添加剂，在氨丙基硅烷化硅胶柱上，用正己烷／异丙醇作流动相，对多种手性化合物进行了高效液相色谱拆分。实验结果表明：用N-十二酰基-L-脯氨酸作添加剂拆分的12种手性化合物，有8种手性化合物能得到拆分，具有较好的手性选择性。虽然用N-十二酰基-L-脯氨酸-3，5-二甲基苯胺作添加剂，手性选择性也较好，但由于含有苯环，紫外吸收增强，基线波动严重。
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| 关 键 词： | N-十二酰基-L-脯氨酸 N-十二酰基-L-脯氨酸-3 5-二甲基苯胺 高效液相色谱 流动相添加剂 |
| 收稿时间： | 2005-10-19 |
| 修稿时间： | 2005-12-26 |
Separation of Enantiomers by High Performance Liquid Chromatography Using Derivants of L-Proline as Chiral Mobile Phase Additives |
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| Chang Yinxia, Hou Zhilin, Li Qiwan, Gao Tianrong, Yuan Liming. Separation of Enantiomers by High Performance Liquid Chromatography Using Derivants of L-Proline as Chiral Mobile Phase Additives[J]. Chinese Journal of Analytical Chemistry, 2006, 34(9): 100-104 |
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| Authors: | Chang Yinxia Hou Zhilin Li Qiwan Gao Tianrong Yuan Liming |
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| Affiliation: | 1 School of Chemistry and Chemical Science, Yunnan Normal University, Kunming 650092; 2 School of Chemistry and Material Science, Shanxi Teachers University, Linfen 041000; 3 Institute of Quality Standards and Testing Technology, Yunnan Academy of Agricultural Sciences, Kunming 650223 |
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| Abstract: | N-dodecanoyl-L-proline and N-dodecanoyl-L-proline-3,5-dimethylanilide derived from L-proline were used as chiral mobile phase additives. Enantiomers were separated using hexane and isopropanol as mobile phase on a aminopropyl silica gel column by high performance liquid chromatography. The results show that the enantioselectivity of N-dodecanoyl-L-proline is better and eight enantiomers of the twelve enantiomers can be separated. The enantioselectivity of N-dodecanoyl-L-proline-3,5-dimethyl-anilide is also good, but the basic line fluctuates strongly due to containing benzene ring in its molecular structure. |
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| Keywords: | N-Dodecanoyl-L-proline N-dodecanoyl-L-proline-3 5-dimethylanilide high performance liquid chromatography mobile phase addition |
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