Arabidopsis camelliol C synthase evolved from enzymes that make pentacycles |
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Authors: | Kolesnikova Mariya D Wilson William K Lynch David A Obermeyer Allie C Matsuda Seiichi P T |
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Affiliation: | Department of Chemistry, Rice University, Houston, Texas 77005, USA. |
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Abstract: | We establish by heterologous expression that the Arabidopsis thaliana oxidosqualene cyclase At1g78955 (CAMS1) makes camelliol C (98%), achilleol A (2%), and beta-amyrin (0.2%). CAMS1 is the first characterized cyclase that generates predominantly a monocyclic triterpene alcohol. Phylogenetic analysis shows that CAMS1 evolved from enzymes that make pentacycles, thus revealing that its pentacyclic beta-amyrin byproduct is an evolutionary relic. Sequence alignments support prior suggestions that decreased steric bulk at a key active-site residue promotes monocycle formation. |
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