利用酵母细胞生物催化合成2-苯乙醇

比较了13个酵母菌株催化合成2-苯乙醇的活性,确定酿酒酵母菌株的催化活性较高.通过单因素和正交实验对酵母催化合成2-苯乙醇的培养基组成进行了优化,采用优化后的催化合成培养基,2-苯乙醇浓度可达4.654g/L,摩尔产率为0.63.实验进一步采用Box-Behnken中心组合设计和响应面分析,建立了2-苯乙醇合成浓度与培养基主要组分之间的二次多项式回归模型,该模型具有较高的准确性,可作为生物催化法合成2-苯乙醇培养基的最优化预测模型.由响应面分析及实验验证得出,在最优培养基条件下,2-苯乙醇浓度可达4.815g/L.

Biocatalytic Synthesis of 2-Phenylethanol by Yeast Cells

The biocatalytic activity of 13 yeast strains was compared for the synthesis of 2-phenylethanol, and a Saccharomyces cerevisiae strain that had high biocatalytic activity was screened out. The experiments of single factor and orthogonal design were carried out to optimize the composition of the medium for the biosynthesis of 2-phenylethanol. The results showed that the concentration of 2-phenylethanol could reach 4.654 g/L with a molar yield of 0.63 in the optimized medium. Furthermore, a model of polynomial regressive equation between the 2-phenylethanol concentration and main components in the medium was established after the Box-Behnken central composite design and response surface methodology were put into practice. The model exhibited fine exactitude and provided a predictive model for optimizing the medium in the biocatalytic synthesis of 2-phenylethanol. Response surface analysis was applied to optimize the medium constituents, and upon applying the optimized medium, the maximum 2-phenylethanol concentration of 4.815 g/L was achieved in testing experiment.