ELF magnetic fields initiate protein tyrosine phosphorylation of the T cell receptor complex |
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| Authors: | Lindström E Still M Mattsson M O Mild K H Luben R A |
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| Institution: | 1. National Institute for Working Life, Umeå, Sweden;2. Department of Surgery, Umeå University, Umeå, Sweden;3. Department of Biology, Örebro University, Örebro, Sweden;4. Department of Biomedical Sciences, University of California, Riverside, CA, USA;1. School of Materials Science and Nanotechnology, Jadavpur University, Kolkata 700032, India;2. Mineral Processing & Technology Research Centre, Department of Metallurgy, Faculty of Engineering & The Built Environment, University of Johannesburg, 2098, South Africa;1. Sate Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing, 210009, China;2. Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing, 210009, China;3. Department of Chemistry, School of Science, China Pharmaceutical University, Nanjing, 211198, China;1. Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell’Insubria, Via J.H. Dunant 3, 21100 Varese, Italy;2. CIBER BBN, C Mariano Esquillor S-N, Zaragoza 50018, Spain;3. Aragón Materials Science Institute (ICMA), Universidad de Zaragoza, CSIC, Campus Río Ebro, Edificio I+D, Mariano Esquillor Gómez, 50018 Zaragoza, Spain;4. University of Zaragoza (UNIZAR), C Mariano Esquillor S-N, Zaragoza 50018, Spain;5. Dipartimento di Chimica & Centro Interdipartimentale “Nanostructured Interfaces and Surfaces” – NIS, Università degli Studi di Torino, Via P. Giuria 7, 10125 Torino, Italy |
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| Abstract: | The human T cell line Jurkat registers a sinusoidal extremely low frequency (ELF), 0.10 mT magnetic fields (MFs) at the level of the plasma membrane. In this study, the protein tyrosine phosphorylation (PY) of two membrane-associated proteins in Jurkat cells were examined following a short-term MFs exposure, the zeta chains and the Src kinases p56lck. These proteins are interesting to study since the earliest biochemical event upon T cell receptor (TcR) activation is PY of the zeta chains. These signalling chains in the TcR complex was assessed using Western blotting and the activation of the p56lck kinase was analysed by in vitro kinase assay. The MFs exposure of Jurkat for 5 min activated p56lck and resulted in PY of zeta. These findings are in line with earlier reports on how MFs exposure affects signal transduction in Jurkat. |
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