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Mg2+与胆汁蛋白质相互作用研究
引用本文:谢安建,姚成立,沈玉华. Mg2+与胆汁蛋白质相互作用研究[J]. 无机化学学报, 2005, 21(3): 413-416
作者姓名:谢安建  姚成立  沈玉华
作者单位:安徽大学化学化工学院,合肥,230039;南京大学配位化学国家重点实验室,南京,210093
基金项目:国家自然科学基金资助项目(No.20031010,20371001)
摘    要:胆汁是一中性溶液,在胆汁固形物中,胆汁酸盐、胆固醇、磷脂、蛋白质、胆红素分别占总质量的65%、3%、20%、5%及1%。蛋白质是胆汁固形物中第四多成分,在胆汁的生理功能的发挥和胆结石的形成中起到重要的作用。研究表明,胆汁蛋白质的主要成分是糖蛋白,主要由胆囊粘膜上皮细胞产生

关 键 词:Mg2+; 胆汁蛋白质; 荧光光谱; 紫外可见光谱
文章编号:1001-4861(2005)03-0413-04
收稿时间:2004-09-01
修稿时间:2004-09-01

Study on the Interaction between Mg2+ and Biliary Protein
XIE An-Jian,YAO Cheng-Li and SHEN Yu-Hua. Study on the Interaction between Mg2+ and Biliary Protein[J]. Chinese Journal of Inorganic Chemistry, 2005, 21(3): 413-416
Authors:XIE An-Jian  YAO Cheng-Li  SHEN Yu-Hua
Affiliation:College of Chemistry and Chemical Engineering, Anhui University, Hefei 230039;State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093,College of Chemistry and Chemical Engineering, Anhui University, Hefei 230039;State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093 and College of Chemistry and Chemical Engineering, Anhui University, Hefei 230039;State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093
Abstract:Biliary protein was isolated and purified by ammonium sulfate fractionation and Sephadex G-75 gel filtration column chromatography. FTIR showed that was a glycoprotein. Its molecular weight was determined as 34.7 kD by MS. The interaction between Mg2+ and biliary protein was studied by fluorescence spectroscopy and UV-Visible spectroscopy. The result showed the quenching mechanism of the combination of Mg2+ with biliary protein was a static quenching procedure. The protein underwent conformation changing and unfolding, more hydrophobic residues would be exposed to solvents after the Mg2+ was added.
Keywords:Mg2+   biliary protein   fluorescence spectroscopy   UV-Visible spectroscopy
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