| Chemically Modified Chitosan Beads as Molecularly Imprinted Polymer Matrix for Adsorptive Separation of Proteins |
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| 作者姓名： | TianYingGUO YongQingXIA GuangJieHAO BangHuaZHANG |
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| 作者单位： | StateKeyLaboratoryofFunctionalPolymerMaterialsforAdsorptionandSeparation,InstituteofPolymerChemistry,NankaiUniversity,Tianjin300071 |
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| 摘 要： | In a phosphate buffer, a hemoglobin (Hb)-imprinted polymer complex was preparedusing maleic anhydride (MAH) modified chitosan beads as matrix, acrylamide (AM) as functionalmonomer, N,N-methylenebisacrylamide (MBA) as cross-linker and potassiumpersulfate (KPS) /sodium hydrogen sulfite (NaHSO3) as initiators. Langmuir analysis showed that an equal class ofadsorption was formed in the molecular imprinting polymer (MIP), and the MIP has highadsorption capacity and selectivity for the imprinted molecule. The MIP can be reused and therecovery was approximately 100% at low concentration.
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| 关 键 词： | 蛋白质 吸附分离 化学修饰壳聚糖 聚丙烯酰胺 分子印迹 |
Chemically Modified Chitosan Beads as Molecularly Imprinted Polymer Matrix for Adsorptive Separation of Proteins |
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| TianYingGUO YongQingXIA GuangJieHAO BangHuaZHANG.Chemically Modified Chitosan Beads as Molecularly Imprinted Polymer Matrix for Adsorptive Separation of Proteins[J].Chinese Chemical Letters,2004,15(11):1339-1341. |
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| Authors: | Tian Ying GUO Yong Qing XIA Guang Jie HAO Bang Hua ZHANG State Key Laboratory of Functional Polymer Materials for Adsorption and Separation |
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| Institution: | Tian Ying GUO*,Yong Qing XIA,Guang Jie HAO,Bang Hua ZHANG State Key Laboratory of Functional Polymer Materials for Adsorption and Separation,Institute of Polymer Chemistry,Nankai University,Tianjin 300071 |
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| Abstract: | In a phosphate buffer, a hemoglobin (Hb)-imprinted polymer complex was prepared using maleic anhydride (MAH) modified chitosan beads as matrix, acrylamide (AM) as functional monomer, N,N-methylenebisacrylamide (MBA) as cross-linker and potassiumpersulfate (KPS) / sodium hydrogen sulfite (NaHSO3) as initiators. Langmuir analysis showed that an equal class of adsorption was formed in the molecular imprinting polymer (MIP), and the MIP has high adsorption capacity and selectivity for the imprinted molecule. The MIP can be reused and the recovery was approximately 100% at low concentration. |
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| Keywords: | Chemically modified chitosan beads polyacrylamide protein imprinting |
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