| 滚环扩增介导的核酸信号放大策略在生物传感器中的应用研究进展 |
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| 引用本文： | 刘巨,梁涛波,许恒毅. 滚环扩增介导的核酸信号放大策略在生物传感器中的应用研究进展[J]. 分析测试学报, 2020, 39(12): 1556-1560 |
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| 作者姓名： | 刘巨 梁涛波 许恒毅 |
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| 作者单位： | 南昌大学食品科学与技术国家重点实验室 |
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| 基金项目： | 江西省主要学科学术和技术带头人培养计划(20194BCJ2204) |
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| 摘 要： | 滚环扩增(RCA)是一种等温核酸扩增技术，因其具有扩增效率高、保真度高等特点，在生物传感器领域得到了广泛的应用。该文介绍了RCA技术的基本原理、RCA环状模板环化方式和RCA的扩增类型，并对基于RCA技术的荧光、比色以及电化学生物传感器进行了介绍，旨在为RCA技术在生物传感器领域的进一步发展和应用提供参考。
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| 关 键 词： | 等温核酸扩增；滚环扩增；生物传感器 |
Advances on Application of Rolling Circle Amplification-mediated Nucleic Acid Signal Amplification Strategy in Biosensors |
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| LIU Ju,LIANG Tao-bo,XU Heng-yi. Advances on Application of Rolling Circle Amplification-mediated Nucleic Acid Signal Amplification Strategy in Biosensors[J]. Journal of Instrumental Analysis, 2020, 39(12): 1556-1560 |
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| Authors: | LIU Ju LIANG Tao-bo XU Heng-yi |
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| Affiliation: | State Key Laboratory of Food Science and Technology,Nanchang University |
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| Abstract: | Rolling circle amplification(RCA) is an isothermal nucleic acid amplification technique.Due to its high efficiency and high fidelity,this technique has been widely used in biosensors.This review introduces the principle of RCA technique,the common cyclization types of RCA template and the common types of RCA,as well as the fluorescent,colorimetric and electrochemical biosensors based on RCA.The aim of this review is to provide references for the further development and application of the RCA technique in biosensors. |
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| Keywords: | isothermal nucleic acid amplification rolling circle amplification biosensor |
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