Biostimulation of HeLa cells by low-intensity visible light |
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Authors: | T I Karu V S Letokhov V V Lobko |
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Institution: | (1) Scientific-Research Center for Technological Lasers, USSR Academic Science, 142092 Troitzk, Moscow Region, USSR;(2) Institute of Spectroscopy USSR Academic Science, 142092 Troitzk, Moscow Region, USSR |
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Abstract: | Summary A culture of HeLa cells was subjected to concurrent and consecutive dichromatic irradiation with visible light of various
wavelengths. Consecutive dichromatic irradiation in the sequence 760 nm+633 nm was found to stimulate the synthesis of DNA
in the cells and that in the sequence 633 nm+760 nm to inhibit it. These effects reached their maxima when the time interval
between the successive irradiation events came to (1⋎3) min. On the strength of these data it is supposed that in HeLa cells
there may exist a photochromic, photoreversible pigment similar to photochrome. Based on the results of dichromatic irradiation
with blue and red light, flavoproteins are being discussed as likely photoreceptors.
Una cultura di cellule HeLa è stata sottoposta ad irradiazione dicromatica concomitante e consecutiva con luce visibile di
varie lunghezze d'onda. Si è trovato che l'irradiazione dicromatica consecutiva nella sequenza 760 nm+633nm stimola la sintesi
del DNA nelle cellule e quella nella sequenza 633 nm+760 nm la inibisce. Questi effetti raggiungono i valori massimi quando
l'intervallo di tempo tra irradiazioni successive arriva a (1⋎3) min. Sulla base di questi dati si suppone che nelle cellule
HeLa possa esistere un pigmento fotocromatico fotoreversibile simile al fotocromo. In base a questi risultati di radiazione
dicromatico con luce blu e rossa, le flavoproteine sono discusse come fotorecettori similari.
Резюме Проведено одновременное и последовательное дихромное облучение клеток НеЛа различными длинами волн видимой области спектра.
Скорость синтеза ДНК стимулируется при последовательном облучении (760+633) нм и инлибирыется при последовательности (633+760)
нм. Эффекты являются максимальными когда интервал между лблучениями составляет (1⋎3) мин. На основе зтих данных предполагается
существование в клетках ХеЛа фотохромното фотообратимого питмента типа фитохрома. На основе данных о дихромном облученим и
красным светом в качестве возможных фоторецепторов обсуждаются флавпротеиды.
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Keywords: | Biological effects of radiation |
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