| Analysis of the Nucleoside Content of Cordyceps sinensis Using the Stepwise Gradient Elution Technique of Thin-Layer Chromatography |
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| 引用本文： | 马敬桦 周福添 吴建勇. Analysis of the Nucleoside Content of Cordyceps sinensis Using the Stepwise Gradient Elution Technique of Thin-Layer Chromatography[J]. 中国化学, 2004, 22(1): 85-91. DOI: 10.1002/cjoc.20040220119 |
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| 作者姓名： | 马敬桦 周福添 吴建勇 |
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| 作者单位： | DepartmentofAppliedBiologyandChemicalTechnology,TheHongKongPolytechnicUniversity,HungHorn,Kowloon,HongKong,China |
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| 基金项目： | the Research Committee of The Hong Kong Polytechnic University (No. G-V877), and the Area of Excellence (AoE) of 揅hinese Medicine Research and Further Development?of the University Grant Council (UGC) of Hong Kong Special Adminis |
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| 摘 要： | Nucleoside is the main class of active components in Cordyceps sinensis. Thin-layer chromatography (TLC) is one of the most commonly used methods in pharmacopoeias for analyzing chemical components of herbal medicine. Since the isocratic elution method cannot be applied successfully in TLC analysis for separating all the nucleoside components, the stepwise gradient elution has been developed in this work to separate eight nucleoside standards with success. In this way, quantitative analyses of the samples of Cordyceps sinensis were achieved via the proposed TLC procedure coupled with the scanning densitometric techniques of CAMAG and TLCQA methods for qualitative and quantitative analysis.
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| 关 键 词： | 薄膜色层法 化学分析 核苷 阶梯洗提 定量分析 |
Analysis of the Nucleoside Content of Cordyceps sinensis Using the Stepwise Gradient Elution Technique of Thin-Layer Chromatography |
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| MA,King-Wah CHAU,Foo-Tim WU,Jian-Yong. Analysis of the Nucleoside Content of Cordyceps sinensis Using the Stepwise Gradient Elution Technique of Thin-Layer Chromatography[J]. Chinese Journal of Chemistry, 2004, 22(1): 85-91. DOI: 10.1002/cjoc.20040220119 |
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| Authors: | MA King-Wah CHAU Foo-Tim WU Jian-Yong |
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| Affiliation: | MA,King-Wah CHAU,Foo-Tim WU,Jian-Yong Department of Applied Biology and Chemical Technology,The Hong Kong Polytechnic University,Hung Hom,Kowloon,Hong Kong,China |
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| Abstract: | Nucleoside is the main class of active components in Cordyceps sinensis. Thin-layer chromatography (TLC) is one of the most commonly used methods in pharmacopoeias for analyzing chemical components of herbal medicine. Since the isocratic elution method cannot be applied successfully in TLC analysis for separating all the nucleoside components, the stepwise gradient elution has been developed in this work to separate eight nucleoside standards with success. In this way, quantitative analyses of the samples of Cordyceps sinensis were achieved via the pro-posed TLC procedure coupled with the scanning densitometric techniques of CAMAG and TLCQA methods for qualitative and quantitative analysis. |
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| Keywords: | thin-layer chromatography Cordyceps sinensis stepwise gradient elution nucleoside software quantitative analysis |
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