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正交试验法优化女贞子提取工艺
引用本文:高爽,王玉真,薄福民,张丽,孙越,杨山景,李凌军.正交试验法优化女贞子提取工艺[J].山东科学,2019,32(4):8-15.
作者姓名:高爽  王玉真  薄福民  张丽  孙越  杨山景  李凌军
作者单位:山东中医药大学药学院,山东 济南 250355
基金项目:国家科技重大专项(重大新药创制)(2017ZX09301064)
摘    要:以特女贞苷、齐墩果酸、熊果酸的提取转移率为指标,采用单因素考察及正交试验法优化女贞子的最佳提取工艺。结果表明,最佳提取工艺为8倍量60%乙醇,提取3次,每次3 h。特女贞苷、齐墩果酸、熊果酸转移率分别为75.42%、93.86%、99.83%。优化所得工艺提取效果良好,工艺简便,可用于工业化生产。

关 键 词:女贞子  特女贞苷  齐墩果酸  熊果酸  提取工艺  
收稿时间:2019-03-26

Optimization of the extraction process for Fructus Ligustri Lucidi using orthogonal experimental design
GAO Shuang,WANG Yu-zhen,BO Fu-min,ZHANG Li,SUN Yue,YANG Shan-jing,LI Ling-jun.Optimization of the extraction process for Fructus Ligustri Lucidi using orthogonal experimental design[J].Shandong Science,2019,32(4):8-15.
Authors:GAO Shuang  WANG Yu-zhen  BO Fu-min  ZHANG Li  SUN Yue  YANG Shan-jing  LI Ling-jun
Institution:School of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China
Abstract:Based on the extraction and transfer rates of specnuezhenide,oleanolic acid,and ursolic acid as indicators,the best extraction process for Fructus Ligustri Lucidi was deduced and optimized using single-factor investigation and an orthogonal experimental design.Results revealed that the optimum extraction process involved the use of eight-fold 60% ethanol,3-fold extraction,and 3-hour duration per round of extraction.The transfer rates of specnuezhenide,oleanolic acid,and ursolic acid were 75.42%,93.86%,and 99.83% respectively.The optimized extraction process features desirable extraction effects and a simple procedure,which can be efficiently used for industrial production.
Keywords:Fructus Ligustri Lucidi  specnuezhenide  oleanolic acid  ursolic acid  extraction process  
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