| Growth Inhibition Effect of Parthenolide on Human Hepatocellular Carcinomacell Line BEL-7402 |
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| 作者姓名： | SONG Xiao-kai ZHANG Wen |
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| 作者单位： | SONG Xiao-kai and ZHANG Wen **College of Biology and Chemical Engineering，Tianjin Institute of Technology，Tianjin 300191，P. R. China |
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| 摘 要： | Introduction Recently,sesquiterpenelactones(SLs)derived fromMexicanIndiamedicinalplantsandEuropeanfe verfewhaveraisedconsiderableinterestbecauseoftheir anti inflammatoryandcomplexpharmacologicalac tions1,2].HerbscontainingSLsarefrequentlyusedby MexicanIn…
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| 关 键 词： | 生长抑制作用 人类 肝癌细胞 PCNA 形态学 |
| 文章编号： | 1005-9040（2006）-02-173-04 |
| 收稿时间： | 2006-01-18 |
Growth Inhibition Effect of Parthenolide on Human Hepatocellular Carcinomacell Line BEL-7402 |
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| SONG Xiao-kai ZHANG Wen.Growth Inhibition Effect of Parthenolide on Human Hepatocellular Carcinomacell Line BEL-7402[J].Chemical Research in Chinese University,2006,22(2):173-176. |
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| Authors: | Xiao-kai SONG Wen ZHANG |
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| Institution: | aCollege of Biology and Chemical Engineering, Tianjin Institute of Technology, Tianjin 300191, P. R. China |
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| Abstract: | To study the effects of parthenolide on human hepatocellular carcinoma cell line BEL-7402 and explore its possible mechanism, human hepatocellular carcinoma cell line BEL-7402 was cultured in vitro and treated with parthenolide of different concentrations. Cells without addition of parthenolide were used as control. The growth of inhibition of cells induced by various concentrations was analyzed by using the MTT assay. The morphologic changes of apoptosis was observed under an inversion microscope by Giemsa staining. The expression levels of PCNA albumen were measured by means of immunohistochemical methods. Parthenolide can inhibit the proliferation of BEL-7402 cells in vitro in adose-dependent manner. Apoptosis with nuclear chromatin condensation, fragmentation and cell shrinkage were found by means of inversion microscopy. Formation of apoptotic bodies was observed by Giemsa staining. The immunohistochemical results show that the expression of PCNA has been decreased. Parthenolide can inhibit the tumor growing of BEL-7402 cells, and induce the apoptosis of cells. The mechanisms of those functions may be via inhibiting the expression of PCNA. |
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| Keywords: | Parthenolide Human hepatocellular carcinomacell line PCNA |
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